The Human CA12 (Carbonic Anhydrase XII) CLIA Kit is a reliable and accurate tool designed for the quantitative measurement of CA12 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for your research needs.Carbonic Anhydrase XII (CA12) is an enzyme that plays a crucial role in maintaining the body's acid-base balance and regulating pH levels. Abnormal expression of CA12 has been linked to various diseases, including cancer, kidney disorders, and metabolic disorders, making it a valuable biomarker for studying these conditions and potential therapeutic targets.
The Human CA12 CLIA Kit is an essential tool for researchers studying the role of CA12 in disease pathogenesis and exploring its potential as a diagnostic and therapeutic target. With its reliable performance and ease of use, this kit is suitable for a wide range of research applications in the fields of biomedicine, oncology, and molecular biology.
Product Name:
Human CA12 (Carbonic Anhydrase XII) CLIA Kit
SKU:
HUES00465
Target:
Human CA12 (Carbonic Anhydrase XII)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CA12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CA12. You can calculate the concentration of Human CA12 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
88-100
94-110
Average (%)
95
94
100
1:4
Range (%)
87-101
93-107
86-98
Average (%)
92
99
93
1:8
Range (%)
99-111
100-118
100-111
Average (%)
105
108
105
1:16
Range (%)
91-108
90-104
98-116
Average (%)
99
95
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
85-99
92
Cell culture media (n=5)
89-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
188.46
452.17
1616.46
183.05
457.51
1483.26
Standard deviation
23.75
50.91
148.39
17.52
48.36
101.01
C V (%)
12.6
11.26
9.18
9.57
10.57
6.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CA12 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CA12 in samples. No significant cross-reactivity or interference between Human CA12 and analogues was observed.
