The Human CA125 (Carbohydrate Antigen 125) CLIA Kit is a cutting-edge diagnostic tool designed for the precise and reliable detection of CA125 levels in human samples such as serum and plasma. CA125 is a well-known tumor marker that is commonly elevated in ovarian cancer and other gynecological malignancies.This CLIA kit offers exceptional sensitivity and specificity, delivering accurate and reproducible results for researchers and clinicians alike. By measuring CA125 levels, this kit can aid in the early detection, monitoring, and management of ovarian cancer, as well as in assessing response to treatment and disease progression.
With its user-friendly design and innovative technology, the Human CA125 CLIA Kit is an invaluable tool for advancing research in oncology and improving patient outcomes. Get your hands on this kit today and take a step towards enhancing cancer diagnostics and treatment.
Product Name:
Human CA125 (Carbohydrate Antigen 125) CLIA Kit
SKU:
HUES00421
Target:
Human CA125 (Carbohydrate Antigen 125)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 IU/mL
Detection range:
0.31-20 IU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CA125. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA125 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA125, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CA125. You can calculate the concentration of Human CA125 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
94-106
97-111
Average (%)
94
99
105
1:4
Range (%)
96-110
85-100
91-106
Average (%)
104
91
96
1:8
Range (%)
93-109
89-102
87-101
Average (%)
100
96
93
1:16
Range (%)
88-101
89-100
93-110
Average (%)
95
95
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
93-110
100
Cell culture media (n=5)
88-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.07
2.71
9.89
1.17
2.78
9.4
Standard deviation
0.09
0.23
0.68
0.11
0.28
0.85
C V (%)
8.41
8.49
6.88
9.4
10.07
9.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CA125 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CA125 in samples. No significant cross-reactivity or interference between Human CA125 and analogues was observed.
