The Human CA125 (Carbohydrate Antigen 125) ELISA Kit is specifically designed for the precise measurement of CA125 levels in human serum, plasma, and cell culture supernatants. With its high level of sensitivity and specificity, this kit ensures accurate and consistent results, making it a valuable tool for a variety of research purposes.CA125 is a protein that is commonly used as a biomarker for ovarian cancer and other gynecological diseases. It is also known to be elevated in conditions such as endometriosis, uterine fibroids, and pelvic inflammatory disease.
The ability to accurately measure CA125 levels can aid in early detection, prognosis, and monitoring of these conditions.Overall, the Human CA125 ELISA Kit is essential for researchers and clinicians looking to study and understand the role of CA125 in disease development and progression, ultimately leading to better diagnostic and therapeutic strategies.
Product Name:
Human CA125 (Carbohydrate Antigen 125) ELISA Kit
SKU:
HUES01799
Target:
Human CA125 (Carbohydrate Antigen 125)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 IU/mL
Detection range:
3.13-200 IU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CA125. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA125 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA125, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CA125. You can calculate the concentration of Human CA125 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
88-101
91-105
Average (%)
101
95
98
1:4
Range (%)
92-106
80-94
83-93
Average (%)
97
86
89
1:8
Range (%)
90-105
85-97
82-93
Average (%)
98
90
87
1:16
Range (%)
92-109
84-97
82-95
Average (%)
99
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
87-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.97
28.07
97.3
10.19
28.72
93.55
Standard deviation
0.6
1.25
3.36
0.64
1.39
3.58
C V (%)
6.02
4.45
3.45
6.28
4.84
3.83
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CA125 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CA125 in samples. No significant cross-reactivity or interference between Human CA125 and analogues was observed.
