The Human CA19-9 (Carbohydrate Antigen 19-9) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of CA19-9 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for researchers looking to study CA19-9 as a biomarker in various conditions. CA19-9 is a carbohydrate antigen that has been associated with a variety of cancers, particularly pancreatic cancer. Monitoring CA19-9 levels can aid in cancer diagnosis, prognosis, and treatment effectiveness.
Additionally, elevated levels of CA19-9 have been linked to other gastrointestinal and hepatobiliary disorders, making it a valuable marker for researchers studying these conditions. With its high sensitivity and specificity, the Human CA19-9 CLIA Kit from Assay Genie is a valuable resource for researchers looking to advance their understanding of CA19-9 and its role in disease progression. Order your kit today to start exploring the potential applications of this important biomarker.
Product Name:
Human CA19-9 (Carbohydrate antigen19-9) CLIA Kit
SKU:
HUES00422
Target:
Human CA19-9 (Carbohydrate antigen19-9)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 IU/mL
Detection range:
0.63-40 IU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CA19-9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA19-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA19-9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CA19-9. You can calculate the concentration of Human CA19-9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
101-118
89-104
Average (%)
105
109
97
1:4
Range (%)
96-110
99-115
99-113
Average (%)
102
108
107
1:8
Range (%)
89-100
84-96
96-110
Average (%)
95
91
103
1:16
Range (%)
101-115
88-102
96-108
Average (%)
108
94
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
104
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.14
3.6
17.05
2.31
3.5
17.2
Standard deviation
0.21
0.28
1.46
0.19
0.37
1.73
C V (%)
9.81
7.78
8.56
8.23
10.57
10.06
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CA19-9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CA19-9 in samples. No significant cross-reactivity or interference between Human CA19-9 and analogues was observed.
