The Human CA19-9 (Carbohydrate Antigen 19-9) ELISA Kit is specifically designed for the quantitative measurement of CA19-9 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and precise results for various research applications.CA19-9 is a tumor-associated antigen often overexpressed in various cancers, particularly pancreatic, colorectal, and gastric cancers.
Monitoring CA19-9 levels can aid in early cancer detection, prognosis, and treatment monitoring, making it a valuable biomarker in oncology research.With its reliable performance, the Human CA19-9 ELISA Kit is a valuable tool for researchers studying cancer biology, diagnostics, and therapy development. Get accurate and reproducible results with this high-quality kit from Assay Genie.
Product Name:
Human CA19-9 (Carbohydrate antigen19-9) ELISA Kit
SKU:
HUES01800
Target:
Human CA19-9 (Carbohydrate antigen19-9)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 IU/mL
Detection range:
6.25-400 IU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CA19-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA19-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA19-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CA19-9. You can calculate the concentration of Human CA19-9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
95-112
85-98
Average (%)
97
103
92
1:4
Range (%)
88-104
82-93
89-101
Average (%)
95
87
94
1:8
Range (%)
86-99
82-95
87-99
Average (%)
93
87
93
1:16
Range (%)
86-101
79-91
88-102
Average (%)
93
86
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
94
EDTA plasma (n=5)
93-107
101
Cell culture media (n=5)
92-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.87
55.99
183.1
22.7
57.61
169.68
Standard deviation
1.35
2.5
6.04
1.21
3.26
8.7
C V (%)
6.47
4.47
3.3
5.33
5.66
5.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CA19-9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CA19-9 in samples. No significant cross-reactivity or interference between Human CA19-9 and analogues was observed.
