Human Cannabinoid Receptor 1, Brain (CNR1) ELISA Kit
The Human Cannabinoid Receptor 1 (Brain CNR1) ELISA Kit is specifically designed for the accurate detection of CNR1 levels in human brain tissue samples. This kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for studying the expression of CNR1 in the brain.CNR1, also known as the cannabinoid receptor 1, is a key receptor in the endocannabinoid system that plays a crucial role in various physiological processes, including pain sensation, appetite regulation, and memory.
Dysregulation of CNR1 has been implicated in neurological disorders such as epilepsy, schizophrenia, and Alzheimer's disease, highlighting the importance of studying CNR1 expression in the brain.By using the Human Cannabinoid Receptor 1 (Brain CNR1) ELISA Kit, researchers can gain valuable insights into the role of CNR1 in brain function and pathology, ultimately aiding in the development of targeted therapies for neurological conditions.
Product Name:
Human Cannabinoid Receptor 1, Brain (CNR1) ELISA Kit
Product Code:
HUDL00608
Size:
96 Assays
Target:
CNR1
Synonyms:
CANN6, CB-R, CB1, CB1A, CB1K5, CNR
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
