The Human Caps Calcyphosine CLIA Kit (HUES00211) is a reliable and precise assay designed for the detection of caps calcyphosine levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Caps calcyphosine is a key protein involved in cellular processes, particularly in the regulation of calcium homeostasis and cell signaling pathways. Dysregulation of caps calcyphosine has been linked to various diseases including cancer, cardiovascular disorders, and neurodegenerative conditions, highlighting its significance as a potential biomarker for disease progression and therapeutic development.
With its advanced technology and user-friendly design, the Human Caps Calcyphosine CLIA Kit (HUES00211) provides researchers with a valuable tool to investigate the role of caps calcyphosine in health and disease, ultimately contributing to the advancement of scientific knowledge and medical treatments.
Product Name:
Human CAPS (Calcyphosine) CLIA Kit
SKU:
HUES00211
Target:
Human CAPS (Calcyphosine)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CAPS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CAPS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CAPS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CAPS. You can calculate the concentration of Human CAPS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
87-100
92-105
Average (%)
101
92
98
1:4
Range (%)
90-101
88-101
98-111
Average (%)
96
95
104
1:8
Range (%)
86-98
86-100
98-114
Average (%)
92
92
105
1:16
Range (%)
99-112
90-105
84-100
Average (%)
106
97
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
99
EDTA plasma (n=5)
102-115
107
Cell culture media (n=5)
93-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.19
300.82
712.88
96.92
270.83
763.91
Standard deviation
11.49
25.15
63.95
7.94
26.05
63.1
C V (%)
12.07
8.36
8.97
8.19
9.62
8.26
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CAPS concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human CAPS in samples. No significant cross-reactivity or interference between Human CAPS and analogues was observed.
