Description
Product Name: | Human Carbohydrate Antigen 125 ELISA Kit |
SKU: | HDES0110 |
Size: | 96T |
Target: | CA125 |
Detection Method: | Sandwich ELISA |
Result Type: | Quantitative: Sensitivity 98.8%, Specificity 99.6% |
Sample Type: | Serum |
Assay Time: | 1 h 15 min |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Indirect-ELISA as the principle to detect the Tuberculosis (TB) IgG in human serum, www.vetassay-elab.com 2 The content of CA125 in serum was determined by enzyme-linked immunosandwich method and avidin and biotin system. Firstly, avidin was coated with microplates to prepare the fixation enzyme, and then biotin-labeled antibodies and calibrators or serum to be tested were added. After incubation and washing, horseradish peroxide-labeled mab was added to form the complex of avidin-biotin-labeled MAB -[CA125-enzyme-labeled mab]. After color development, OD value was determined by enzyme-labeled MAB. The concentration - absorbance curve was fitted by computer or drawing, and the content of CA125 in serum was calculated inversely.