Human Carbonic Anhydrase 2 Recombinant Protein (RPPB1459)
- SKU:
- RPPB1459
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P00918
- Research Area:
- Enzymes
Description
| Product Name: | Human Carbonic Anhydrase 2 Recombinant Protein |
| Product Code: | RPPB1459 |
| Size: | 20µg |
| Species: | Human |
| Target: | Carbonic Anhydrase 2 |
| Synonyms: | Carbonic anhydrase 2, Carbonate dehydratase 2, Carbonic Anhydrase II, CA-II, Carbonic anhydrase C, CAC, CA2, CAII, Car2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | Carbonic Anhydrase 2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 50mM NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK |
| Biological Activity: | Specific activity is 50-70 nmoles/min/�g and was obtained by measuring the increase in the amount of p-nitrophenol by its esterase activity. Specific activity is defined as the amount of�p-nitrophenol that 1ug of enzyme can reduce at 25C for 1 minute. |
The enzyme Carbonic anhydrase II having an accession number of NP_414668 is also called carbonate dehydratase which is part of the enzyme family that catalyses rapid inter-conversion of carbon dioxide & water to bicarbonate, carbonic acid and protons (CO2 + H2O= HCO3 + H+), a reaction that occurs rather slowly in the absence of a catalyst. The majority of carbonic anhydrases enclose a zinc ion in their active site and therefore is classified as metalloenzymes.The most important function of Carbonic anhydrase is known to preserve acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Carbonic anhydrase has 3 different classes: alpha, beta and gamma which share very little sequence or structural similarity, thus far they all perform the same function and require a zinc ion at the active site. Mammalian carbonic anhydrase is monomeric and belongs to the alpha class. Plant carbonic anhydrase is dimeric and belongs to the beta class.Methane-producing bacteria carbonic anhydrase is trimeric and grows in hot springs which forms the gamma class.
Carbonic anhydrase 2 Human Recombinant protein produced in E.Coli containing 260 amino acids (1-260) and having a molecular mass of 29.2 kDa. The Carbonic anhydrase 2 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family. |
| UniProt Protein Details: | Protein type:Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen Chromosomal Location of Human Ortholog: 8q22 Cellular Component: extracellular space; microvillus; apical part of cell; axon; basolateral plasma membrane; cytoplasm; plasma membrane; cytosol; myelin sheath Molecular Function:protein binding; carbonate dehydratase activity; zinc ion binding Biological Process: secretion; positive regulation of osteoclast differentiation; positive regulation of synaptic transmission, GABAergic; positive regulation of cellular pH reduction; one-carbon compound metabolic process; odontogenesis of dentine-containing teeth; response to zinc ion; bicarbonate transport; response to estrogen stimulus; regulation of intracellular pH; positive regulation of bone resorption; morphogenesis of an epithelium; kidney development; response to pH Disease: Osteopetrosis, Autosomal Recessive 3 |
| NCBI Summary: | The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] |
| UniProt Code: | P00918 |
| NCBI GenInfo Identifier: | 115456 |
| NCBI Gene ID: | 760 |
| NCBI Accession: | P00918.2 |
| UniProt Secondary Accession: | P00918,Q6FI12, Q96ET9, B2R7G8, |
| UniProt Related Accession: | P00918 |
| Molecular Weight: | 260 |
| NCBI Full Name: | Carbonic anhydrase 2 |
| NCBI Synonym Full Names: | carbonic anhydrase II |
| NCBI Official Symbol: | CA2�� |
| NCBI Official Synonym Symbols: | CAC; CAII; Car2; CA-II; HEL-76�� |
| NCBI Protein Information: | carbonic anhydrase 2; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; carbonate dehydratase II; epididymis luminal protein 76 |
| UniProt Protein Name: | Carbonic anhydrase 2 |
| UniProt Synonym Protein Names: | Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II |
| Protein Family: | Beta-carotene hydroxylase |
| UniProt Gene Name: | CA2�� |
| UniProt Entry Name: | CAH2_HUMAN |
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