The Human CD45RO Polyclonal Antibody is a valuable tool for researchers studying the CD45RO protein, a cell surface marker associated with memory T cells. This antibody is raised in rabbits and has been validated for use in Western blot applications. It specifically binds to the CD45RO antigen, allowing for detection and analysis in various human cell types.CD45RO is important in immune responses, particularly in the memory T cell subset which plays a key role in adaptive immunity.
Research involving CD45RO can provide insights into immune memory, autoimmune diseases, and infectious diseases. The Human CD45RO Polyclonal Antibody is a reliable reagent for investigating the function and expression of CD45RO in immunology and disease research.
Product Name:
Human CD45RO Polyclonal Antibody
SKU:
CAB24838
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 27-120 of human CD45RO(NP_563578.2).
Sequence:
SPTP SPTG LTTA KMPS VPLS SDPL PTHT TAFS PAST FERE NDFS ETTT SLSP DNTS TQVS PDSL DNAS AFNT TGVS SVQT PHLP THAD SQTP SA
Membrane, Membrane raft, Single-pass type I membrane protein.
Calculated MW:
130kDa/147kDa
Observed MW:
Refer to figures
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Purification Method:
Affinity purification
Gene ID:
5788
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Immunofluorescence analysis of paraffin-embedded Rat spleen using Human CD45RO Rabbit pAb(CAB24838) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
![PerCP Anti-Human CD45RO Antibody [UCHL1] (AGEL1699)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/22230/606449/percp-anti-human-cd45ro-antibody-uchl1-agel1699__54156.1707498062.jpg?c=2 "PerCP Anti-Human CD45RO Antibody [UCHL1] (AGEL1699)")
![APC Anti-Human CD45RO Antibody [UCHL1] (AGEL1698)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/237558/618238/apc-anti-human-cd45ro-antibody-uchl1-agel1698__92818.1732238363.jpg?c=2 "APC Anti-Human CD45RO Antibody [UCHL1] (AGEL1698)")
![PE Anti-Human CD45RO Antibody [UCHL1] (AGEL1697)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/22228/606655/pe-anti-human-cd45ro-antibody-uchl1-agel1697__01098.1707498688.jpg?c=2 "PE Anti-Human CD45RO Antibody [UCHL1] (AGEL1697)")
![Purified Anti-Human CD45RO Antibody [UCHL1] (AGEL3509)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/221630/605408/anti-human-cd45ro-antibody-uchl1-agel3509__09035.1707494809.jpg?c=2 "Purified Anti-Human CD45RO Antibody [UCHL1] (AGEL3509)")
![FITC Anti-Human CD45RO Antibody [UCHL1] (AGEL1696)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/22227/605601/fitc-anti-human-cd45ro-antibody-uchl1-agel1696__65188.1707495405.jpg?c=2 "FITC Anti-Human CD45RO Antibody [UCHL1] (AGEL1696)")