The Human CEA (Carcinoembryonic Antigen) CLIA Kit is specifically designed for the precise detection of Carcinoembryonic Antigen levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for a variety of research applications.Carcinoembryonic Antigen is a critical biomarker associated with various cancers, particularly in colorectal cancer.
Monitoring CEA levels can provide valuable information on disease progression, prognosis, and response to treatment. With the ability to detect CEA levels in a reliable and reproducible manner, this kit is an essential tool for researchers studying cancer biology and developing innovative therapeutic strategies.
Product Name:
Human CEA (Carcinoembryonic Antigen) CLIA Kit
SKU:
HUES00426
Target:
Human CEA (Carcinoembryonic Antigen)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CEA/CD66. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CEA/CD66 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CEA/CD66, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CEA/CD66. You can calculate the concentration of Human CEA/CD66 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
94-107
98-112
Average (%)
101
100
106
1:4
Range (%)
97-111
93-106
92-105
Average (%)
105
99
98
1:8
Range (%)
95-108
97-109
84-96
Average (%)
102
104
91
1:16
Range (%)
87-103
88-102
87-101
Average (%)
94
96
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
105
EDTA plasma (n=5)
94-105
99
Cell culture media (n=5)
95-108
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.37
87.73
407.8
45.36
90.58
371.72
Standard deviation
4.11
6.27
29.36
4.89
6.99
24.53
C V (%)
8.86
7.15
7.2
10.78
7.72
6.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CEA/CD66 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CEA/CD66 in samples. No significant cross-reactivity or interference between Human CEA/CD66 and analogues was observed.
