The Human CEA (Carcinoembryonic Antigen) ELISA Kit is designed for the quantitative measurement of CEA levels in various biological samples of human origin. CEA is a glycoprotein that serves as a tumor marker, particularly associated with gastrointestinal cancers such as colorectal carcinoma. Elevations in CEA levels can indicate disease progression, making it a valuable biomarker for cancer diagnosis, monitoring therapeutic responses, and detecting cancer recurrence. Accurate detection and quantification of CEA are essential for oncological research, clinical diagnostics, and patient management. This ELISA kit offers exceptional specificity and sensitivity, ensuring precise and reproducible results. Manufactured under stringent quality control measures, the kit provides reliable performance and user-friendly protocols. Choose Assay Genie's CEA ELISA Kit for robust and dependable quantification of this significant tumor marker in your studies and clinical applications.
Product Name:
Human CEA (Carcinoembryonic Antigen) ELISA Kit
SKU:
AEES00083
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
