The Human CFB (Complement Factor B) ELISA Kit is a specialized assay designed to quantitatively detect CFB levels in a variety of biological samples. Complement Factor B is a crucial component of the alternative complement pathway involved in immune responses, complement activation, and the clearance of pathogens.
It plays a pivotal role in bridging innate and adaptive immunity by participating in opsonization, inflammation, and immune complex clearance. Understanding CFB levels is essential for deciphering immune system function, complement activation, and disease processes. Accurate measurement of CFB is vital for investigating its role in immune responses and disease pathogenesis. With exceptional sensitivity and specificity, Assay Genie's CFB ELISA Kit ensures precise and reproducible results. Manufactured under strict quality control guidelines, this kit delivers reliable performance and user-friendly operation, making it an excellent choice for both laboratory research and clinical applications. Researchers can trust Assay Genie's CFB ELISA Kit for dependable quantitation of this essential complement factor in their studies.
Product Name:
Human CFB (Complement Factor B) ELISA Kit
SKU:
AEES00092
Target:
Human CFB (Complement Factor B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CFB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CFB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CFB. You can calculate the concentration of Human CFB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
86-99
90-100
Average (%)
103
93
97
1:4
Range (%)
94-104
87-100
95-108
Average (%)
98
92
102
1:8
Range (%)
96-110
96-107
84-96
Average (%)
102
101
92
1:16
Range (%)
99-108
90-106
87-101
Average (%)
102
98
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-107
104
EDTA plasma (n=5)
98-107
103
Cell culture media (n=5)
98-109
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.81
2.18
8.88
0.84
2.03
9.2
Standard deviation
0.05
0.1
0.46
0.04
0.1
0.44
C V (%)
6.17
4.59
5.18
4.76
4.93
4.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CFB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CFB in samples. No significant cross-reactivity or interference between Human CFB and analogues was observed.
