Human CG alpha (Chorionic Gonadotropin Alpha Polypeptide) CLIA Kit
The Human CG Alpha (Chorionic Gonadotropin Alpha Polypeptide) CLIA Kit is specifically designed for the precise measurement of CG Alpha levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.CG Alpha is an important protein involved in pregnancy and fertility, as it plays a key role in maintaining the corpus luteum and supporting early pregnancy. Abnormal levels of CG Alpha can indicate potential issues with pregnancy or certain health conditions, making it a valuable biomarker for diagnosis and monitoring.
With its advanced technology and reliable performance, the Human CG Alpha CLIA Kit is an essential tool for researchers and healthcare professionals studying reproductive health, pregnancy, and related disorders. Trust in this kit for dependable results and deep insights into CG Alpha levels in human biological samples.
Product Name:
Human CG alpha (Chorionic Gonadotropin Alpha Polypeptide) CLIA Kit
SKU:
HUES00251
Target:
Human CG alpha (Chorionic Gonadotropin Alpha Polypeptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CGα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CGα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CGα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CGα. You can calculate the concentration of Human CGα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
85-101
89-101
Average (%)
94
92
96
1:4
Range (%)
94-109
95-112
94-109
Average (%)
102
102
102
1:8
Range (%)
96-113
102-118
100-113
Average (%)
104
110
107
1:16
Range (%)
84-97
88-104
99-113
Average (%)
91
95
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
101
EDTA plasma (n=5)
83-95
90
Cell culture media (n=5)
92-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.83
157.13
366.66
52.43
158.27
339.99
Standard deviation
6.33
15.76
39.78
4.77
17.87
35.32
C V (%)
12.96
10.03
10.85
9.1
11.29
10.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CGα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CGα in samples. No significant cross-reactivity or interference between Human CGα and analogues was observed.
