The Human CG (Chorionic Gonadotropin) CLIA Kit is a cutting-edge assay designed for the precise detection of CG levels in human serum, plasma, and cell culture supernatants. This advanced kit offers unmatched sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Chorionic Gonadotropin is a hormone important in pregnancy, being produced by the placenta and playing a key role in supporting the development of the fetus.
Abnormal levels of CG can indicate various reproductive health issues, making it a valuable biomarker for studying fertility, gestational conditions, and more.With its reliable performance and user-friendly design, the Human CG CLIA Kit is an indispensable tool for researchers and healthcare professionals looking to explore the intricate mechanisms of reproductive health and pregnancy-related disorders.
Product Name:
Human CG (Chorionic Gonadotropin) CLIA Kit
SKU:
HUES00150
Target:
Human CG (Chorionic Gonadotropin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CG. You can calculate the concentration of Human CG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-116
102-115
92-106
Average (%)
107
108
98
1:4
Range (%)
92-104
95-110
94-111
Average (%)
97
103
101
1:8
Range (%)
91-105
102-116
86-99
Average (%)
98
108
91
1:16
Range (%)
96-114
103-117
98-114
Average (%)
104
109
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
96-111
102
Cell culture media (n=5)
97-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.87
3.53
18.27
1.98
3.82
18.35
Standard deviation
0.21
0.4
1.57
0.22
0.43
1.55
C V (%)
11.23
11.33
8.59
11.11
11.26
8.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CG in samples. No significant cross-reactivity or interference between Human CG and analogues was observed.
