Human CGRP (Calcitonin Gene Related Peptide) CLIA Kit
The Human CGRP (Calcitonin Gene-Related Peptide) CLIA Kit is specifically designed for the accurate detection of CGRP levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.CGRP is a key neuropeptide involved in pain modulation, inflammation, and vasodilation. It plays a crucial role in conditions like migraine, chronic pain, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and exploring potential treatment options.
The Human CGRP CLIA Kit from Assay Genie provides researchers with a reliable tool to quantify CGRP levels and gain valuable insights into the role of this neuropeptide in various physiological and pathological processes.
Product Name:
Human CGRP (Calcitonin Gene Related Peptide) CLIA Kit
SKU:
HUES00409
Target:
Human CGRP (Calcitonin Gene Related Peptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CGRP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CGRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CGRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CGRP1. You can calculate the concentration of Human CGRP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
93-106
99-113
Average (%)
92
99
105
1:4
Range (%)
85-100
87-100
102-119
Average (%)
91
93
109
1:8
Range (%)
85-97
91-103
92-106
Average (%)
92
98
97
1:16
Range (%)
90-103
91-103
88-104
Average (%)
95
97
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.09
67.96
243.94
25.95
74.01
258.52
Standard deviation
3.06
5.48
26.03
2.44
8.27
27.87
C V (%)
11.3
8.06
10.67
9.4
11.17
10.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CGRP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CGRP1 in samples. No significant cross-reactivity or interference between Human CGRP1 and analogues was observed.
