The Human CHGB (Chromogranin B) CLIA Kit is specifically designed for the precise detection of chromogranin B levels in human serum, plasma, and cell culture supernatants. This advanced kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable tool for a variety of research applications.Chromogranin B is a vital protein involved in regulating hormone and neurotransmitter release, playing a crucial role in various physiological processes. Research suggests that abnormalities in chromogranin B levels may be linked to conditions such as cardiovascular diseases, endocrine disorders, and neuroendocrine tumors, highlighting its significance as a key biomarker for studying these conditions and potentially developing targeted therapies.
The Human CHGB CLIA Kit from AssayGenie provides researchers with a reliable and efficient solution for measuring chromogranin B levels, allowing for in-depth investigations and advancements in the fields of endocrinology, neurology, and oncology. Stay ahead in your research with this innovative kit from AssayGenie.
Product Name:
Human CHGB (Chromogranin B) CLIA Kit
SKU:
HUES00509
Target:
Human CHGB (Chromogranin B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CHGB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CHGB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CHGB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CHGB. You can calculate the concentration of Human CHGB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
89-104
92-104
Average (%)
92
95
97
1:4
Range (%)
96-109
97-115
88-100
Average (%)
103
105
94
1:8
Range (%)
96-109
97-115
87-98
Average (%)
103
105
92
1:16
Range (%)
89-102
100-116
102-117
Average (%)
96
106
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
96-110
103
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.97
152.86
409.56
53.12
142.5
382.11
Standard deviation
6.31
14.95
44.31
4.65
11.81
39.97
C V (%)
12.63
9.78
10.82
8.75
8.29
10.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CHGB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CHGB in samples. No significant cross-reactivity or interference between Human CHGB and analogues was observed.
