The Human CK-18 (KRT18) Cytokeratin-18 CLIA Kit is a cutting-edge assay designed for the precise quantification of cytokeratin-18 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Cytokeratin-18, a major intermediate filament protein, is a key marker for epithelial cells and is crucial for maintaining cellular structure and function.
Abnormal levels of cytokeratin-18 have been linked to various diseases and conditions such as liver disease, cancer, and apoptosis, making it a valuable biomarker for exploring disease pathogenesis and developing targeted therapies.With its advanced technology and high-performance capabilities, the Human CK-18 Cytokeratin-18 CLIA Kit is an essential tool for researchers and clinicians alike seeking to unravel the intricate mechanisms of disease and improve patient outcomes.
Product Name:
Human CK-18/KRT18 (Cytokeratin 18) CLIA Kit
SKU:
HUES01105
Target:
Human CK-18/KRT18 (Cytokeratin 18)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CK-18/KRT18. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CK-18/KRT18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CK-18/KRT18, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CK-18/KRT18. You can calculate the concentration of Human CK-18/KRT18 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
88-101
98-109
Average (%)
107
95
104
1:4
Range (%)
93-108
101-116
91-106
Average (%)
100
106
98
1:8
Range (%)
91-104
103-120
96-113
Average (%)
98
110
104
1:16
Range (%)
94-108
89-103
101-114
Average (%)
100
95
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
105
EDTA plasma (n=5)
100-116
106
Cell culture media (n=5)
92-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.7
166.93
877.03
93.16
160.51
887.6
Standard deviation
8.98
18.56
75.6
10.3
15.92
63.73
C V (%)
9.1
11.12
8.62
11.06
9.92
7.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CK-18/KRT18 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CK-18/KRT18 in samples. No significant cross-reactivity or interference between Human CK-18/KRT18 and analogues was observed.
