Human CKMB (Creatine Kinase MB Isoenzyme) CLIA Kit
The Human CKMB (Creatine Kinase MB Isoenzyme) CLIA Kit is a reliable and accurate tool for detecting CKMB levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing consistent and reproducible results for a variety of research applications.CKMB is an important biomarker for assessing myocardial infarction and cardiac muscle damage. Monitoring CKMB levels can aid in the diagnosis and treatment of heart conditions, helping healthcare professionals make informed decisions about patient care.
With its advanced technology and precise detection capabilities, the Human CKMB CLIA Kit is a valuable resource for researchers and clinicians studying cardiovascular health and developing new therapeutic interventions. Trust in this kit for dependable results and meaningful insights into CKMB levels in human biological samples.
Product Name:
Human CKMB (Creatine Kinase MB Isoenzyme) CLIA Kit
SKU:
HUES00813
Target:
Human CKMB (Creatine Kinase MB Isoenzyme)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CKMB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CKMB. You can calculate the concentration of Human CKMB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
96-112
102-118
Average (%)
98
103
108
1:4
Range (%)
94-107
99-115
90-101
Average (%)
99
107
95
1:8
Range (%)
90-104
90-107
84-95
Average (%)
95
98
90
1:16
Range (%)
86-97
99-115
103-118
Average (%)
92
106
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
101
EDTA plasma (n=5)
96-108
103
Cell culture media (n=5)
96-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.44
171.8
774.47
103.54
182.74
796.95
Standard deviation
10.67
15.87
86.35
8.45
19.9
65.03
C V (%)
11.06
9.24
11.15
8.16
10.89
8.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CKMB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CKMB in samples. No significant cross-reactivity or interference between Human CKMB and analogues was observed.
