Human CKMB (Creatine Kinase MB Isoenzyme) ELISA Kit
The Human CK-MB (Creatine Kinase-MB Isoenzyme) ELISA Kit is a cutting-edge tool designed for the precise quantification of CK-MB levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results that are vital for various research applications.CK-MB is an essential enzyme found predominantly in heart muscles, making it a crucial biomarker for diagnosing and monitoring myocardial infarction (heart attack). Elevated levels of CK-MB in the bloodstream indicate damage to the heart muscle, providing valuable insights into cardiac health and potential treatment options.
By utilizing the Human CK-MB ELISA Kit, researchers can gain valuable information on cardiac health, improve diagnostic accuracy, and contribute to the development of innovative therapies for heart-related conditions. With its reliable performance and ease of use, this kit is a valuable asset for researchers in the field of cardiology and cardiovascular medicine.
Product Name:
Human CKMB (Creatine Kinase MB Isoenzyme) ELISA Kit
SKU:
HUES02470
Target:
Human CKMB (Creatine Kinase MB Isoenzyme)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CKMB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CKMB. You can calculate the concentration of Human CKMB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
94-109
92-107
Average (%)
93
101
99
1:4
Range (%)
86-99
95-111
90-103
Average (%)
93
102
96
1:8
Range (%)
86-98
95-109
93-105
Average (%)
91
103
98
1:16
Range (%)
85-101
97-110
95-110
Average (%)
92
102
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
92
EDTA plasma (n=5)
83-96
90
Cell culture media (n=5)
94-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.2
15.19
35.83
4.87
15.14
37.32
Standard deviation
0.3
0.61
1.83
0.28
0.8
1.49
C V (%)
5.79
4.02
5.12
5.83
5.26
3.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CKMB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CKMB in samples. No significant cross-reactivity or interference between Human CKMB and analogues was observed.
