Human CLC (Charcot Leyden Crystal Protein) CLIA Kit
The Human CLC (Charcot-Leyden Crystal) Protein CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of CLC protein levels in human samples, including serum, plasma, and cell culture supernatants. This innovative kit offers reliable and reproducible results, making it an invaluable tool for a variety of research applications.The CLC protein, also known as Charcot-Leyden crystal galectin, is associated with eosinophilic inflammation and is a marker for eosinophil activation and degranulation. Elevated levels of CLC protein have been linked to allergic conditions, asthma, and parasitic infections, making it a key biomarker for the study of these diseases and potential therapeutic interventions.
With its high sensitivity and precision, the Human CLC Protein CLIA Kit provides researchers with a powerful tool for investigating the role of CLC protein in inflammatory processes and developing targeted treatments for related disorders. Order yours today and unlock new insights into the complex mechanisms of eosinophilic inflammation.
Product Name:
Human CLC (Charcot Leyden Crystal Protein) CLIA Kit
SKU:
HUES00863
Target:
Human CLC (Charcot Leyden Crystal Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CLC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CLC. You can calculate the concentration of Human CLC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
87-99
86-101
Average (%)
91
92
93
1:4
Range (%)
94-107
86-99
100-114
Average (%)
102
93
108
1:8
Range (%)
93-104
86-97
100-118
Average (%)
98
91
109
1:16
Range (%)
87-98
87-101
99-114
Average (%)
93
94
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
94-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.59
225.87
935.82
112.07
246.17
927.38
Standard deviation
13.28
24.73
102.1
9.84
22.25
81.24
C V (%)
12.94
10.95
10.91
8.78
9.04
8.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CLC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLC in samples. No significant cross-reactivity or interference between Human CLC and analogues was observed.
