The Human CLU (Clusterin) CLIA Kit is a highly reliable and sensitive assay designed for the accurate quantification of clusterin levels in human serum, plasma, and cell culture supernatants. Clusterin, also known as apolipoprotein J, is a versatile glycoprotein that plays various roles in cell survival, apoptosis, and tissue remodeling.This CLIA kit offers high specificity and precision, ensuring accurate and reproducible results for your research needs.
Clusterin is implicated in a wide range of diseases, including Alzheimer's disease, cancer, and kidney disorders, making it a valuable biomarker for understanding disease mechanisms and developing potential therapeutic interventions.Whether investigating disease pathways or exploring potential treatment options, the Human CLU (Clusterin) CLIA Kit is a powerful tool for researchers looking to uncover the role of clusterin in health and disease.
Product Name:
Human CLU (Clusterin) CLIA Kit
SKU:
HUES00037
Target:
Human CLU (Clusterin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CLU. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLU, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CLU. You can calculate the concentration of Human CLU in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-118
92-104
94-105
Average (%)
107
98
99
1:4
Range (%)
97-110
93-108
86-99
Average (%)
103
99
91
1:8
Range (%)
89-103
98-114
99-114
Average (%)
94
104
105
1:16
Range (%)
92-103
100-119
100-116
Average (%)
98
108
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
100
EDTA plasma (n=5)
95-110
101
Cell culture media (n=5)
99-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.68
7.54
20.12
2.57
7.38
21.21
Standard deviation
0.24
0.64
1.96
0.22
0.81
1.98
C V (%)
8.96
8.49
9.74
8.56
10.98
9.34
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CLU concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLU in samples. No significant cross-reactivity or interference between Human CLU and analogues was observed.
