The Human Clusterin ELISA Kit (HUES01325) from Assay Genie is an essential tool for accurately measuring clusterin levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results for a variety of research applications.Clusterin, also known as apolipoprotein J, is a versatile protein involved in various physiological and pathological processes, including inflammation, lipid transportation, and cell survival.
Its dysregulation has been linked to diseases such as Alzheimer's, cancer, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.Trust in the Human Clusterin ELISA Kit from Assay Genie to help advance your research efforts and gain valuable insights into the role of clusterin in health and disease. Visit www.assaygenie.com/human-clu-clusterin-elisa-kit-hues01325/ to learn more and order your kit today.
Product Name:
Human CLU (Clusterin) ELISA Kit
SKU:
HUES01325
Target:
Human CLU (Clusterin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CLU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CLU. You can calculate the concentration of Human CLU in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
96-109
85-100
Average (%)
100
102
92
1:4
Range (%)
89-105
81-92
86-101
Average (%)
97
86
94
1:8
Range (%)
90-105
84-96
85-100
Average (%)
96
89
91
1:16
Range (%)
89-103
81-91
86-98
Average (%)
94
87
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
91-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.73
19.44
92.85
11.07
19.22
88.8
Standard deviation
0.62
0.83
3.0
0.58
0.96
4.37
C V (%)
5.78
4.27
3.23
5.24
4.99
4.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CLU concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CLU in samples. No significant cross-reactivity or interference between Human CLU and analogues was observed.
