Human CNR1 (Cannabinoid Receptor 1, Brain) CLIA Kit
The Human CNR1 (Cannabinoid Receptor 1) Brain CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of CNR1 levels in human brain tissue samples. This kit provides researchers with a valuable tool for studying the role of the cannabinoid receptor in various neurological processes.The CNR1 receptor plays a critical role in the endocannabinoid system, which is involved in regulating various physiological functions such as pain sensation, mood, memory, and appetite. Dysregulation of the endocannabinoid system has been implicated in a range of neurological disorders, including epilepsy, schizophrenia, and neurodegenerative diseases.
With its high sensitivity and specificity, the Human CNR1 Brain CLIA Kit offers researchers the ability to precisely quantify CNR1 levels, enabling a better understanding of the receptor's function in the brain and its potential implications for disease pathogenesis. This kit is an invaluable tool for advancing research in the field of cannabinoid receptor biology and neurological disorders.
Product Name:
Human CNR1 (Cannabinoid Receptor 1, Brain) CLIA Kit
SKU:
HUES00420
Target:
Human CNR1 (Cannabinoid Receptor 1, Brain)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CNR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CNR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CNR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CNR1. You can calculate the concentration of Human CNR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
88-104
102-119
Average (%)
98
95
109
1:4
Range (%)
87-98
100-119
94-105
Average (%)
92
108
100
1:8
Range (%)
94-108
97-114
98-115
Average (%)
99
104
106
1:16
Range (%)
99-114
92-106
90-101
Average (%)
105
97
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
93-106
99
Cell culture media (n=5)
97-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.58
241.95
857.53
92.17
236.52
805.01
Standard deviation
10.49
26.4
77.26
8.23
28.22
65.61
C V (%)
10.33
10.91
9.01
8.93
11.93
8.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CNR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CNR1 in samples. No significant cross-reactivity or interference between Human CNR1 and analogues was observed.
