The Human CNTN1 (Contactin-1) CLIA Kit is a powerful tool for researchers looking to accurately measure levels of Contactin-1 in human samples. Contactin-1 is a cell adhesion molecule that plays a key role in the development and function of the nervous system, making it a valuable biomarker for studying neurodevelopmental disorders and neurological diseases.This CLIA kit offers high sensitivity and specificity, ensuring precise and reliable results in serum, plasma, and cell culture supernatants.
With its easy-to-use protocol and quick assay time, researchers can efficiently analyze Contactin-1 levels in a variety of sample types, providing valuable insights into the role of this protein in health and disease.Whether investigating potential therapeutic targets for neurological conditions or studying the impact of Contactin-1 dysregulation, the Human CNTN1 CLIA Kit is a valuable resource for advancing research in neuroscience and beyond.
Product Name:
Human CNTN1 (Contactin 1) CLIA Kit
SKU:
HUES00853
Target:
Human CNTN1 (Contactin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CNTN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CNTN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CNTN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CNTN1. You can calculate the concentration of Human CNTN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
88-103
93-106
Average (%)
106
96
98
1:4
Range (%)
100-115
92-103
99-111
Average (%)
108
98
105
1:8
Range (%)
95-110
100-113
94-110
Average (%)
102
106
100
1:16
Range (%)
94-109
92-106
96-111
Average (%)
101
99
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
96-110
104
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.77
177.48
912.28
105.68
165.58
922.43
Standard deviation
9.06
18.51
91.78
12.12
14.79
75.36
C V (%)
8.41
10.43
10.06
11.47
8.93
8.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CNTN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CNTN1 in samples. No significant cross-reactivity or interference between Human CNTN1 and analogues was observed.
