The Human COL10 (Collagen Type X) CLIA Kit is specifically designed for the quantitative measurement of Collagen Type X levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reliable results for a variety of research applications.Collagen Type X, a specialized type of collagen found in cartilage, plays a crucial role in skeletal development and maintenance.
Abnormal levels of Collagen Type X have been associated with various musculoskeletal disorders, making it a valuable biomarker for studying these conditions and exploring potential treatment options.With its user-friendly protocol and robust performance, the Human COL10 CLIA Kit is an essential tool for researchers and healthcare professionals seeking to better understand the role of Collagen Type X in health and disease.
Product Name:
Human COL10 (Collagen Type X) CLIA Kit
SKU:
HUES00493
Target:
Human COL10 (Collagen Type X)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL10. You can calculate the concentration of Human COL10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
92-104
93-107
Average (%)
97
97
99
1:4
Range (%)
101-114
88-102
88-102
Average (%)
108
93
94
1:8
Range (%)
94-111
98-114
89-102
Average (%)
101
104
96
1:16
Range (%)
99-113
93-110
93-107
Average (%)
104
101
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
95-112
102
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.82
76.72
344.8
47.71
77.17
374.85
Standard deviation
4.99
7.84
28.31
5.46
8.52
23.65
C V (%)
11.39
10.22
8.21
11.44
11.04
6.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL10 in samples. No significant cross-reactivity or interference between Human COL10 and analogues was observed.
