Human COL11 alpha1 (Collagen Type XI Alpha 1) CLIA Kit
The Human COL11A1 (Collagen Type XI Alpha 1) CLIA Kit is a highly reliable and accurate assay designed for the detection of COL11A1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.COL11A1 is a key component of collagen type XI, playing a crucial role in maintaining the structure and function of tissues such as cartilage and intervertebral discs.
Abnormal levels of COL11A1 have been implicated in various musculoskeletal disorders, making it a valuable biomarker for understanding and potentially treating these conditions.By utilizing the Human COL11A1 CLIA Kit, researchers can gain valuable insights into the role of COL11A1 in health and disease, paving the way for advancements in musculoskeletal research and potential therapeutic interventions.
Product Name:
Human COL11 alpha1 (Collagen Type XI Alpha 1) CLIA Kit
SKU:
HUES00494
Target:
Human COL11 alpha1 (Collagen Type XI Alpha 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL11α1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL11α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL11α1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL11α1. You can calculate the concentration of Human COL11α1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
84-100
94-109
Average (%)
93
91
100
1:4
Range (%)
88-103
85-99
92-107
Average (%)
95
92
98
1:8
Range (%)
90-103
95-109
94-106
Average (%)
96
101
99
1:16
Range (%)
87-98
102-116
93-109
Average (%)
92
109
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-110
102
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.42
150.62
479.24
54.81
162.35
526.35
Standard deviation
6.07
15.23
48.59
5.95
12.99
53.9
C V (%)
11.8
10.11
10.14
10.86
8.0
10.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL11α1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL11α1 in samples. No significant cross-reactivity or interference between Human COL11α1 and analogues was observed.
