The Human COL14 (Collagen Type 14) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of collagen type 14 levels in human samples. This kit provides accurate and reliable results, thanks to its high sensitivity and specificity, making it suitable for a variety of research purposes.Collagen type 14 is a vital component of the extracellular matrix, playing a crucial role in tissue development, wound healing, and cell adhesion. Abnormal levels of collagen type 14 have been linked to various diseases and conditions, including fibrosis, arthritis, and skin disorders.
Therefore, measuring collagen type 14 levels can provide valuable insights into disease progression and potential therapeutic interventions.With the Human COL14 CLIA Kit, researchers can effectively study the role of collagen type 14 in various health conditions and explore its potential as a therapeutic target. This kit offers a reliable and efficient solution for accurate collagen type 14 detection, making it a valuable tool for advancing scientific research and clinical diagnostics.
Product Name:
Human COL14 (Collagen Type 14) CLIA Kit
SKU:
HUES00527
Target:
Human COL14 (Collagen Type 14)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL14. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL14, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL14. You can calculate the concentration of Human COL14 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
87-98
98-111
Average (%)
107
92
103
1:4
Range (%)
100-113
88-103
88-100
Average (%)
107
96
94
1:8
Range (%)
102-116
104-116
93-106
Average (%)
107
110
99
1:16
Range (%)
99-111
92-103
101-116
Average (%)
105
98
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
95-106
100
Cell culture media (n=5)
87-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.16
197.0
896.62
105.11
203.89
841.72
Standard deviation
11.62
14.74
98.9
11.85
24.14
72.14
C V (%)
11.72
7.48
11.03
11.27
11.84
8.57
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL14 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL14 in samples. No significant cross-reactivity or interference between Human COL14 and analogues was observed.
