Human COL4 alpha1 (Collagen Type IV Alpha 1) ELISA Kit
The Human COL4 alpha1 (Collagen Type IV Alpha 1) ELISA Kit is a reliable tool for the quantitative detection of COL4 alpha1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and consistent results for various research applications.Collagen Type IV Alpha 1 is a key component of the basement membrane, playing a crucial role in maintaining tissue structure and barrier function. Dysregulation of COL4 alpha1 has been associated with various diseases, including Alport syndrome, diabetic nephropathy, and certain types of cancer.
Therefore, this ELISA kit is valuable for studying these conditions and exploring potential therapeutic interventions.Overall, the Human COL4 alpha1 ELISA Kit from Assay Genie provides researchers with a powerful tool to investigate the role of collagen type IV alpha 1 in health and disease, facilitating valuable insights for future medical advancements.
Product Name:
Human COL4 alpha1 (Collagen Type IV Alpha 1) ELISA Kit
SKU:
HUES01917
Target:
Human COL4 alpha1 (Collagen Type IV Alpha 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL4α1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL4α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL4α1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL4α1. You can calculate the concentration of Human COL4α1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
96-112
98-110
Average (%)
104
103
104
1:4
Range (%)
90-104
87-97
86-100
Average (%)
96
92
92
1:8
Range (%)
93-108
86-96
87-103
Average (%)
99
91
94
1:16
Range (%)
90-105
86-102
83-93
Average (%)
97
93
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
93-108
101
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.07
2.74
7.57
1.16
2.93
7.95
Standard deviation
0.06
0.16
0.25
0.08
0.17
0.3
C V (%)
5.61
5.84
3.3
6.9
5.8
3.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COL4α1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL4α1 in samples. No significant cross-reactivity or interference between Human COL4α1 and analogues was observed.
