Human COL6 alpha1 (Collagen Type VI Alpha 1) CLIA Kit
The Human COL6 Alpha1 (Collagen Type VI Alpha 1) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of COL6 Alpha1 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.Collagen Type VI Alpha 1 is a key component of the extracellular matrix and is essential for maintaining tissue structure and integrity. Dysregulation of COL6 Alpha1 has been implicated in various diseases, including muscular dystrophies, connective tissue disorders, and skin conditions.
Therefore, this kit is a valuable tool for studying the role of COL6 Alpha1 in these conditions and exploring potential therapeutic interventions.Overall, the Human COL6 Alpha1 CLIA Kit offers researchers a reliable method for quantifying COL6 Alpha1 levels in human samples, providing valuable insights into its role in health and disease.
Product Name:
Human COL6 alpha1 (Collagen Type VI Alpha 1) CLIA Kit
SKU:
HUES00490
Target:
Human COL6 alpha1 (Collagen Type VI Alpha 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL6α1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL6α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL6α1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL6α1. You can calculate the concentration of Human COL6α1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
94-108
92-106
Average (%)
95
99
98
1:4
Range (%)
93-107
90-108
101-115
Average (%)
101
98
109
1:8
Range (%)
96-113
89-102
99-114
Average (%)
103
96
106
1:16
Range (%)
101-116
88-100
95-107
Average (%)
107
94
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
99-113
106
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.01
248.29
922.9
90.68
262.07
959.04
Standard deviation
7.94
21.05
86.94
8.34
24.4
60.32
C V (%)
8.45
8.48
9.42
9.2
9.31
6.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL6α1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL6α1 in samples. No significant cross-reactivity or interference between Human COL6α1 and analogues was observed.
