Human COL6 alpha3 (Collagen Type VI Alpha 3) CLIA Kit
The Human COL6 (Collagen Type VI) Alpha 3 CLIA Kit is specifically designed for the quantification of Collagen Type VI Alpha 3 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.Collagen Type VI Alpha 3 is a key component of the extracellular matrix and is essential for maintaining tissue structure and integrity. Dysregulation of Collagen Type VI Alpha 3 has been implicated in various diseases including musculoskeletal disorders, skin diseases, and fibrotic conditions.
Therefore, this CLIA kit serves as a valuable tool for studying the role of Collagen Type VI Alpha 3 in disease pathogenesis and for developing potential diagnostics and therapies.Overall, the Human COL6 Alpha 3 CLIA Kit provides researchers with a powerful tool for studying the biology of Collagen Type VI Alpha 3 and its implications in human health and disease.
Product Name:
Human COL6 alpha3 (Collagen Type VI Alpha 3) CLIA Kit
SKU:
HUES00525
Target:
Human COL6 alpha3 (Collagen Type VI Alpha 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL6α3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL6α3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL6α3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL6α3. You can calculate the concentration of Human COL6α3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
92-109
87-98
Average (%)
94
100
92
1:4
Range (%)
88-100
91-108
99-113
Average (%)
93
98
105
1:8
Range (%)
102-118
96-109
98-110
Average (%)
108
102
103
1:16
Range (%)
93-106
86-97
90-104
Average (%)
99
91
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
107
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
91-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.73
150.54
490.98
44.06
163.9
468.93
Standard deviation
4.29
12.0
33.44
5.15
11.9
31.28
C V (%)
9.38
7.97
6.81
11.69
7.26
6.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL6α3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL6α3 in samples. No significant cross-reactivity or interference between Human COL6α3 and analogues was observed.
