Human COL6 alpha3 (Collagen Type VI Alpha 3) ELISA Kit
The Human COL6 Alpha3 (Collagen Type VI Alpha 3) ELISA Kit is specifically designed for the precise measurement of collagen type VI alpha 3 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for a variety of research applications.Collagen type VI alpha 3 is a key component of the extracellular matrix and is essential for maintaining tissue structure and integrity. Dysregulation of collagen type VI alpha 3 has been implicated in various diseases, including muscular dystrophy, skin disorders, and connective tissue disorders.
Therefore, this ELISA kit is essential for studying the role of collagen type VI alpha 3 in these conditions and investigating potential therapeutic interventions.Overall, the Human COL6 Alpha3 ELISA Kit offers researchers a reliable and efficient method for quantifying collagen type VI alpha 3 levels, providing valuable insights into the pathophysiology of diseases related to extracellular matrix dysfunction.
Product Name:
Human COL6 alpha3 (Collagen Type VI Alpha 3) ELISA Kit
SKU:
HUES01998
Target:
Human COL6 alpha3 (Collagen Type VI Alpha 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL6α3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL6α3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL6α3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL6α3. You can calculate the concentration of Human COL6α3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
89-102
96-108
Average (%)
91
95
101
1:4
Range (%)
89-104
81-93
86-98
Average (%)
95
87
92
1:8
Range (%)
90-102
84-94
85-98
Average (%)
97
89
91
1:16
Range (%)
92-108
86-98
83-95
Average (%)
99
91
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
90
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
93-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.31
180.87
947.14
105.56
195.68
1004.44
Standard deviation
6.09
7.78
50.67
6.35
8.45
30.13
C V (%)
6.26
4.3
5.35
6.02
4.32
3.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COL6α3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL6α3 in samples. No significant cross-reactivity or interference between Human COL6α3 and analogues was observed.
