Human COL8 alpha1 (Collagen Type VIII Alpha 1) CLIA Kit
The Human COL8-alpha1 (Collagen Type VIII Alpha 1) CLIA Kit is a highly reliable and sensitive assay designed for the precise detection of COL8-alpha1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an essential tool for research in various fields.COL8-alpha1 is a crucial component of collagen type VIII, which plays a significant role in maintaining the structural integrity of blood vessels and tissues.
Dysregulation of COL8-alpha1 has been implicated in various diseases, including ocular disorders, cardiovascular diseases, and connective tissue disorders. Therefore, this CLIA kit is essential for studying the role of COL8-alpha1 in these conditions and developing targeted therapeutic interventions.
Product Name:
Human COL8 alpha1 (Collagen Type VIII Alpha 1) CLIA Kit
SKU:
HUES00492
Target:
Human COL8 alpha1 (Collagen Type VIII Alpha 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL8α1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL8α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL8α1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL8α1. You can calculate the concentration of Human COL8α1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
92-105
86-101
Average (%)
105
97
93
1:4
Range (%)
98-114
86-101
95-108
Average (%)
105
93
103
1:8
Range (%)
88-100
91-102
86-97
Average (%)
94
97
91
1:16
Range (%)
86-98
90-103
96-113
Average (%)
93
96
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
96-110
104
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.42
223.65
899.53
86.94
214.98
838.57
Standard deviation
8.09
20.11
96.88
7.97
16.02
72.45
C V (%)
8.57
8.99
10.77
9.17
7.45
8.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL8α1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL8α1 in samples. No significant cross-reactivity or interference between Human COL8α1 and analogues was observed.
