Human COL9 alpha3 (Collagen Type IX Alpha 3) CLIA Kit
The Human COL9 Alpha3 (Collagen Type IX Alpha 3) CLIA Kit is a specialized assay designed for the accurate quantification of COL9 Alpha3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.COL9 Alpha3 is a critical component of Collagen Type IX, which plays a crucial role in maintaining the structural integrity of cartilage and other connective tissues. Abnormalities in COL9 Alpha3 levels have been associated with disorders such as osteoarthritis and other joint-related conditions.
Thus, this CLIA kit serves as an invaluable tool for researchers studying these pathologies and exploring potential therapeutic interventions.Overall, the Human COL9 Alpha3 CLIA Kit is a reliable and efficient solution for researchers seeking to investigate the role of COL9 Alpha3 in physiological processes and disease states. Its high performance and user-friendly design make it an ideal choice for laboratories conducting research in the field of connective tissue biology and musculoskeletal disorders.
Product Name:
Human COL9 alpha3 (Collagen Type IX Alpha 3) CLIA Kit
SKU:
HUES00551
Target:
Human COL9 alpha3 (Collagen Type IX Alpha 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL9α3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL9α3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL9α3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL9α3. You can calculate the concentration of Human COL9α3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
96-113
90-104
Average (%)
91
104
95
1:4
Range (%)
98-112
94-108
100-113
Average (%)
104
100
106
1:8
Range (%)
88-102
98-114
92-103
Average (%)
93
105
97
1:16
Range (%)
98-114
101-116
102-116
Average (%)
106
106
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
95
EDTA plasma (n=5)
89-104
96
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
0.97
4.14
0.53
1.06
4.06
Standard deviation
0.06
0.11
0.35
0.07
0.09
0.43
C V (%)
11.76
11.34
8.45
13.21
8.49
10.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL9α3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL9α3 in samples. No significant cross-reactivity or interference between Human COL9α3 and analogues was observed.
