Human Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) ELISA Kit
The Human Cold Shock Domain-Containing Protein E1 (CSDE1) ELISA Kit is designed for the precise measurement of CSDE1 levels in human biological samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.CSDE1 is a key RNA-binding protein that plays a crucial role in post-transcriptional gene regulation, RNA processing, and mRNA stability. Dysregulation of CSDE1 has been associated with various diseases including cancer, neurodevelopmental disorders, and neurodegenerative diseases, highlighting its importance as a potential biomarker for understanding disease mechanisms and developing targeted therapies.
With its high performance and reliability, the Human CSDE1 ELISA Kit is an invaluable tool for researchers studying the role of CSDE1 in health and disease, providing valuable insights into its involvement in various cellular processes and pathological conditions.
Product Name:
Human Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1) ELISA Kit
Product Code:
HUDL00694
Size:
96 Assays
Synonyms:
CDH11, CAD11, OB, OSF4, Cadherin 11 Type 2, OB-Cadherin
Detection Method:
sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
