The Human Collagen Type IV Alpha 1 (COL4A1) ELISA Kit is a valuable tool for the precise measurement of COL4A1 levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring dependable and consistent results that are suitable for various research applications.COL4A1 is a key component of type IV collagen, a major structural protein in the extracellular matrix. It is essential for maintaining tissue integrity and plays a critical role in various biological processes such as cell adhesion, migration, and differentiation.
Dysregulation of COL4A1 has been implicated in several diseases including kidney disease, muscular dystrophy, and certain cancers, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.Overall, the Human Collagen Type IV Alpha 1 (COL4A1) ELISA Kit is a reliable and versatile tool for researchers looking to investigate the role of COL4A1 in health and disease, providing accurate and reproducible results that contribute to advancing scientific knowledge and discovering new treatments.
Product Name:
Human Collagen Type IV Alpha 1 (COL4a1) ELISA Kit
Product Code:
HUDL00625
Size:
96 Assays
Target:
COL4a1
Synonyms:
COL4-A1, Arresten, Collagen Alpha-1(IV)chain
Detection Method:
Sandwich
Range:
0.937-60ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
