Human COMP (Cartilage Oligomeric Matrix Protein) ELISA Kit
The Human COMP (Cartilage Oligomeric Matrix Protein) ELISA Kit is specifically designed for the accurate measurement of COMP levels in human cartilage tissue samples. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.COMP is a key protein found in cartilage, playing a vital role in maintaining cartilage structure and function. Abnormal levels of COMP have been associated with various joint diseases such as osteoarthritis and rheumatoid arthritis, making it a valuable biomarker for studying these conditions and potentially identifying new treatment strategies.
With easy-to-follow protocols and reliable performance, the Human COMP ELISA Kit is a valuable tool for researchers studying cartilage biology and exploring potential therapeutic interventions for cartilage-related disorders.
Product Name:
Human COMP (Cartilage Oligomeric Matrix Protein) ELISA Kit
SKU:
HUES01815
Target:
Human COMP (Cartilage Oligomeric Matrix Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COMP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COMP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COMP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COMP. You can calculate the concentration of Human COMP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
98-113
89-102
Average (%)
96
106
96
1:4
Range (%)
87-102
86-99
87-97
Average (%)
93
93
92
1:8
Range (%)
94-107
85-98
81-92
Average (%)
99
90
87
1:16
Range (%)
87-101
81-93
87-101
Average (%)
94
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
98
EDTA plasma (n=5)
92-104
97
Cell culture media (n=5)
96-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.05
6.13
19.76
2.14
6.1
20.56
Standard deviation
0.14
0.3
1.0
0.12
0.29
0.9
C V (%)
6.83
4.89
5.06
5.61
4.75
4.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COMP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COMP in samples. No significant cross-reactivity or interference between Human COMP and analogues was observed.
