Human Complement Component 1, Q Subcomponent Like Protein 1 (C1qL1) ELISA Kit
The Human Complement Component 1 Q Subcomponent-Like Protein 1 (C1QL1) ELISA Kit is a specialized assay designed for the accurate measurement of C1QL1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.C1QL1 is a protein that belongs to the C1q/TNF-related protein (CTRP) family and plays a crucial role in various biological processes, including synaptic plasticity, neuronal development, and inflammation. Dysregulation of C1QL1 has been linked to conditions such as autism, schizophrenia, and Alzheimer's disease, highlighting its potential as a valuable biomarker for studying and understanding these complex disorders.
By utilizing the Human C1QL1 ELISA Kit, researchers can accurately quantify C1QL1 levels in biological samples, enabling detailed investigations into its role in various physiological and pathological processes. With its high sensitivity and specificity, this kit provides a powerful tool for advancing research in neuroscience, immunology, and related fields.
Product Name:
Human Complement Component 1, Q Subcomponent Like Protein 1 (C1qL1) ELISA Kit
Product Code:
HUDL00369
Size:
96 Assays
Target:
C1qL1
Synonyms:
CRF, C1QRF, CTRP14, C1q-related factor, C1q and tumor necrosis factor-related protein 14
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
