Human Complement Component 1,R Subcomponent (C1r) ELISA Kit
The Human Complement Component 1 R subcomponent (C1r) ELISA Kit is a highly sensitive and specific assay designed for the precise measurement of C1r levels in human serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an essential tool for researchers studying the complement system and its role in various diseases.Complement component 1 R subcomponent (C1r) is a key enzyme in the classical pathway of the complement system, playing a crucial role in immune response and inflammation.
Dysregulation of C1r has been implicated in autoimmune diseases, inflammatory disorders, and other conditions, highlighting its importance as a potential biomarker for disease diagnosis and monitoring.With its high performance and accuracy, the Human Complement Component 1 R subcomponent (C1r) ELISA Kit is an invaluable resource for researchers seeking to better understand the role of C1r in health and disease, ultimately leading to new insights and therapeutic targets for a range of medical conditions.
Product Name:
Human Complement Component 1,R Subcomponent (C1r) ELISA Kit
Product Code:
HUDL00374
Size:
96 Assays
Target:
C1r
Synonyms:
C1-r, complement Component 1, Complement component 1 subcomponent r
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-102
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
