The Human CP (Ceruloplasmin) CLIA Kit is specifically designed for the precise measurement of ceruloplasmin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Ceruloplasmin is an important copper-binding protein that plays a crucial role in iron metabolism and antioxidant defense mechanisms.
Dysregulation of ceruloplasmin levels has been linked to various health conditions such as Wilson's disease, Alzheimer's disease, and cardiovascular disorders, making it a valuable biomarker for studying these diseases and developing potential treatments.Overall, the Human CP (Ceruloplasmin) CLIA Kit is a reliable and efficient tool for researchers looking to investigate the role of ceruloplasmin in health and disease.
Product Name:
Human CP (Ceruloplasmin) CLIA Kit
SKU:
HUES00135
Target:
Human CP (Ceruloplasmin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 μg/mL
Detection range:
0.16-10 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CP. You can calculate the concentration of Human CP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
87-99
84-96
Average (%)
102
93
91
1:4
Range (%)
100-115
89-101
91-103
Average (%)
107
94
98
1:8
Range (%)
87-99
98-113
101-115
Average (%)
93
105
109
1:16
Range (%)
95-112
93-105
87-99
Average (%)
102
99
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-111
105
EDTA plasma (n=5)
90-101
96
Cell culture media (n=5)
95-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.45
3.83
0.54
1.57
3.6
Standard deviation
0.07
0.12
0.38
0.05
0.14
0.23
C V (%)
12.96
8.28
9.92
9.26
8.92
6.39
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CP in samples. No significant cross-reactivity or interference between Human CP and analogues was observed.
