The Human CPP (Copeptin) ELISA Kit is specifically designed for the precise measurement of copeptin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, which makes it a valuable tool for a variety of research purposes.Copeptin is a peptide hormone that is released alongside vasopressin in response to stress and plays a key role in regulating water balance and blood pressure.
Elevated copeptin levels have been associated with various conditions such as heart failure, sepsis, and diabetes, making it a crucial biomarker for studying these diseases and exploring potential treatment options.Overall, the Human CPP (Copeptin) ELISA Kit provides researchers with a reliable and efficient method for studying copeptin levels in human samples, offering valuable insights into its biological functions and potential clinical applications.
Product Name:
Human CPP (Copeptin) ELISA Kit
SKU:
HUES01982
Target:
Human CPP (Copeptin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CPP. During the reaction, CPP in the sample or standard competes with a fixed amount of CPP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CPP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CPP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
88-100
87-98
Average (%)
93
94
92
1:4
Range (%)
84-95
88-100
98-111
Average (%)
89
93
105
1:8
Range (%)
88-101
92-103
93-107
Average (%)
95
98
100
1:16
Range (%)
88-101
90-104
97-108
Average (%)
95
95
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-98
93
EDTA plasma (n=5)
84-96
91
Cell culture media (n=5)
91-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.4
220.1
769.8
112.5
239.3
705.1
Standard deviation
5.5
13.0
34.6
7.2
9.8
25.4
C V (%)
5.22
5.91
4.49
6.4
4.1
3.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of CPP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes CPP in samples. No significant cross-reactivity or interference between CPP and analogues was observed.
