The Human CR2 (CD21) Complement Receptor 2 CLIA Kit is a reliable and sensitive assay designed for the quantitative detection of CR2 levels in human serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it suitable for a variety of research applications.CR2, also known as CD21, is a key protein involved in the complement system, playing a critical role in immune responses and inflammation. Dysregulation of CR2 expression has been linked to various autoimmune disorders, infectious diseases, and immunodeficiencies, highlighting its importance as a potential biomarker for studying these conditions and developing therapeutic interventions.
With its high performance and ease of use, the Human CR2 (CD21) Complement Receptor 2 CLIA Kit is an indispensable tool for researchers looking to investigate the role of CR2 in health and disease.
Product Name:
Human CR2/CD21 (Complement Receptor 2) CLIA Kit
SKU:
HUES00921
Target:
Human CR2/CD21 (Complement Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CR2/CD21. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CR2/CD21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CR2/CD21, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CR2/CD21. You can calculate the concentration of Human CR2/CD21 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
101-115
94-109
Average (%)
106
107
101
1:4
Range (%)
95-109
93-107
86-99
Average (%)
102
100
93
1:8
Range (%)
95-109
86-98
90-103
Average (%)
101
92
96
1:16
Range (%)
87-101
91-106
89-100
Average (%)
95
98
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
90-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
216.32
413.63
1365.6
229.97
429.87
1324.67
Standard deviation
21.52
30.69
110.07
27.96
39.08
146.91
C V (%)
9.95
7.42
8.06
12.16
9.09
11.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CR2/CD21 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CR2/CD21 in samples. No significant cross-reactivity or interference between Human CR2/CD21 and analogues was observed.
