Human CRHBP (Corticotropin Releasing Hormone Bingding Protein) CLIA Kit
The Human CRHBP (Corticotropin-Releasing Hormone Binding Protein) CLIA Kit is a powerful tool for detecting and measuring CRHBP levels in human biological samples. This kit offers high sensitivity and accuracy, allowing for precise and reliable results in serum, plasma, and cell culture supernatants.CRHBP is a key regulator of the stress response system, binding to corticotropin-releasing hormone (CRH) and modulating its activity. Dysregulation of CRHBP has been linked to various stress-related disorders, including anxiety, depression, and PTSD.
The Human CRHBP CLIA Kit provides researchers with the means to investigate the role of CRHBP in these conditions and explore potential therapeutic interventions.With its easy-to-use format and robust performance, the Human CRHBP CLIA Kit is an invaluable tool for studying the complex interplay between CRHBP and stress-related disorders, offering new insights into their pathophysiology and potential treatment options.
Product Name:
Human CRHBP (Corticotropin Releasing Hormone Bingding Protein) CLIA Kit
SKU:
HUES00869
Target:
Human CRHBP (Corticotropin Releasing Hormone Bingding Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CRHBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRHBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRHBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CRHBP. You can calculate the concentration of Human CRHBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
95-112
100-114
Average (%)
101
103
107
1:4
Range (%)
86-98
99-111
92-107
Average (%)
91
106
98
1:8
Range (%)
92-108
96-109
96-109
Average (%)
99
102
101
1:16
Range (%)
89-100
86-99
99-111
Average (%)
94
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
97
EDTA plasma (n=5)
98-114
105
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.66
269.88
678.73
102.1
246.75
628.93
Standard deviation
11.59
22.51
61.56
12.12
18.83
64.4
C V (%)
10.47
8.34
9.07
11.87
7.63
10.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CRHBP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CRHBP in samples. No significant cross-reactivity or interference between Human CRHBP and analogues was observed.
