The Human CRN (Corin) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of corin levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its unparalleled accuracy and specificity, ensuring consistent and dependable results for a variety of research purposes.Corin, also known as Corin is a key enzyme involved in regulating blood pressure and electrolyte balance in the body. Its dysregulation has been linked to various cardiovascular disorders, making it a crucial biomarker for studying these conditions and exploring potential treatment options.
With the Human CRN (Corin) CLIA Kit from Assay Genie, researchers can confidently investigate the role of corin in cardiovascular health and diseases, leading to a deeper understanding of its mechanisms and potential therapeutic interventions. Trust in the quality and precision of Assay Genie's CLIA Kit to advance your research endeavors in the field of cardiovascular health and beyond.
Product Name:
Human CRN (Corin) CLIA Kit
SKU:
HUES00041
Target:
Human CRN (Corin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CRN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CRN. You can calculate the concentration of Human CRN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
90-106
86-99
Average (%)
93
97
91
1:4
Range (%)
95-109
99-117
90-107
Average (%)
102
107
98
1:8
Range (%)
87-100
93-106
91-104
Average (%)
94
100
97
1:16
Range (%)
85-98
86-100
98-116
Average (%)
90
91
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
91
EDTA plasma (n=5)
88-102
93
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.88
220.41
743.13
107.31
210.18
712.16
Standard deviation
9.42
20.32
48.3
11.78
20.14
66.94
C V (%)
8.57
9.22
6.5
10.98
9.58
9.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CRN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CRN in samples. No significant cross-reactivity or interference between Human CRN and analogues was observed.
