The Human CRT (Calreticulin) CLIA Kit is a cutting-edge assay designed for the precise detection of calreticulin levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an indispensable tool for a variety of research applications.Calreticulin is a multifunctional protein that plays a key role in cellular processes such as calcium homeostasis, protein folding, and immune response modulation.
Dysregulation of calreticulin has been implicated in several diseases including cancer, autoimmune disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human CRT CLIA Kit offers researchers a reliable method for analyzing calreticulin expression levels, providing insights into its role in disease pathogenesis and potential therapeutic strategies.
Product Name:
Human CRT (Calreticulin) CLIA Kit
SKU:
HUES00414
Target:
Human CRT (Calreticulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CRT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CRT. You can calculate the concentration of Human CRT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-108
88-99
91-107
Average (%)
102
94
98
1:4
Range (%)
99-113
88-100
97-112
Average (%)
107
93
103
1:8
Range (%)
96-112
93-105
93-107
Average (%)
103
100
101
1:16
Range (%)
89-104
94-108
103-116
Average (%)
95
100
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
89-104
95
Cell culture media (n=5)
95-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.04
296.16
681.4
87.76
271.58
717.76
Standard deviation
9.48
24.85
62.21
7.29
24.28
63.81
C V (%)
10.3
8.39
9.13
8.31
8.94
8.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CRT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CRT in samples. No significant cross-reactivity or interference between Human CRT and analogues was observed.
