The Human Cry AlphaB Crystallin Alpha B CLIA Kit (HUES00865) is a high-quality assay designed for the accurate detection of Cry AlphaB Crystallin Alpha B levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring dependable and reproducible results, making it suitable for a variety of research applications.Cry AlphaB Crystallin Alpha B is a protein that plays a vital role in protecting cells from stress and maintaining cellular integrity. Dysregulation of this protein has been linked to various diseases including neurodegenerative disorders, cardiovascular diseases, and cancer.
Therefore, monitoring Cry AlphaB Crystallin Alpha B levels can provide valuable insights into disease progression and potential therapeutic interventions.Overall, the Human Cry AlphaB Crystallin Alpha B CLIA Kit (HUES00865) is a valuable tool for researchers interested in studying the role of Cry AlphaB Crystallin Alpha B in health and disease, offering reliable and accurate results for downstream analysis.
Product Name:
Human CRY alphaB (Crystallin Alpha B) CLIA Kit
SKU:
HUES00865
Target:
Human CRY alphaB (Crystallin Alpha B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CRYαB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRYαB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRYαB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CRYαB. You can calculate the concentration of Human CRYαB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
89-102
90-102
Average (%)
106
96
96
1:4
Range (%)
89-100
97-109
85-97
Average (%)
94
103
92
1:8
Range (%)
96-111
101-117
102-116
Average (%)
102
107
110
1:16
Range (%)
97-112
83-98
98-112
Average (%)
104
90
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
102
EDTA plasma (n=5)
97-110
103
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.23
188.73
884.48
102.93
178.03
883.63
Standard deviation
9.95
18.0
59.61
12.69
14.62
90.13
C V (%)
9.11
9.54
6.74
12.33
8.21
10.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CRYαB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CRYαB in samples. No significant cross-reactivity or interference between Human CRYαB and analogues was observed.
