The Human CTN-I (TNNI3) Cardiac Troponin I CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of cardiac troponin I levels in human serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it ideal for a wide range of research applications in the field of cardiology.Cardiac troponin I is a key biomarker used in the diagnosis and prognosis of acute coronary syndromes and other cardiovascular diseases.
Elevated levels of cardiac troponin I are indicative of cardiac muscle damage, providing vital information for the assessment and management of patients with heart conditions. This kit enables researchers to study the role of cardiac troponin I in cardiovascular health and disease, facilitating the development of novel diagnostic and therapeutic strategies.
Product Name:
Human cTn-I/TNNI3 (cardiac Troponin I) CLIA Kit
SKU:
HUES00429
Target:
Human cTn-I/TNNI3 (cardiac Troponin I)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human cTn-I/TNNI3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human cTn-I/TNNI3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human cTn-I/TNNI3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human cTn-I/TNNI3. You can calculate the concentration of Human cTn-I/TNNI3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
101-115
94-108
Average (%)
97
106
100
1:4
Range (%)
95-110
86-98
97-111
Average (%)
101
92
102
1:8
Range (%)
85-100
97-113
86-101
Average (%)
91
105
92
1:16
Range (%)
99-116
100-115
84-97
Average (%)
106
108
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
107
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
93-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
254.53
513.41
1903.3
257.46
557.97
1898.33
Standard deviation
24.89
60.02
156.64
30.05
44.3
184.52
C V (%)
9.78
11.69
8.23
11.67
7.94
9.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human cTn-I/TNNI3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human cTn-I/TNNI3 in samples. No significant cross-reactivity or interference between Human cTn-I/TNNI3 and analogues was observed.
