The Human CTSB (Cathepsin B) CLIA Kit is a cutting-edge tool designed for the precise measurement of Cathepsin B levels in human samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit provides researchers with accurate and reproducible results, making it an invaluable asset for a wide range of studies.Cathepsin B is an important enzyme that plays a key role in various physiological processes, including protein degradation, cell migration, and apoptosis.
Dysregulation of Cathepsin B has been linked to numerous diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions, highlighting its significance as a potential biomarker for disease detection and therapeutic development.Whether investigating disease mechanisms, biomarker discovery, or drug development, the Human CTSB CLIA Kit offers a reliable and efficient solution for researchers seeking to unravel the complex role of Cathepsin B in health and disease.
Product Name:
Human CTSB (Cathepsin B) CLIA Kit
SKU:
HUES00018
Target:
Human CTSB (Cathepsin B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CTSB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTSB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTSB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CTSB. You can calculate the concentration of Human CTSB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
86-102
95-111
Average (%)
90
93
101
1:4
Range (%)
97-114
101-115
99-118
Average (%)
104
106
108
1:8
Range (%)
86-98
99-113
93-110
Average (%)
93
106
101
1:16
Range (%)
100-113
91-107
99-113
Average (%)
107
98
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
104
EDTA plasma (n=5)
101-116
108
Cell culture media (n=5)
100-114
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.33
277.7
727.18
92.77
304.35
793.1
Standard deviation
8.14
28.52
62.46
11.96
22.92
89.54
C V (%)
8.11
10.27
8.59
12.89
7.53
11.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CTSB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTSB in samples. No significant cross-reactivity or interference between Human CTSB and analogues was observed.
