The Human CTSB (Cathepsin B) ELISA Kit is meticulously designed to facilitate the precise and quantitative detection of Cathepsin B levels in various human biological samples. Cathepsin B, a member of the papain-like cysteine protease family, performs crucial roles in intracellular protein degradation, extracellular matrix remodeling, and various physiological processes. By accurately quantifying Cathepsin B, researchers can gain valuable insights into its involvement in cellular homeostasis, disease pathogenesis, and therapeutic targets.
Our ELISA kit offers exceptional sensitivity and specificity, ensuring robust and reproducible results vital for elucidating Cathepsin B's diverse functions. Crafted with stringent quality control measures, this kit delivers reliable performance and user-friendly protocols, making it an outstanding choice for cutting-edge research applications. Trust Assay Genie's Cathepsin B ELISA Kit for accurate and dependable quantification of this essential biomarker in your research endeavors.
Product Name:
Human CTSB (Cathepsin B) ELISA Kit
SKU:
AEES00160
Target:
Human CTSB (Cathepsin B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTSB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTSB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTSB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CTSB. You can calculate the concentration of Human CTSB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-100
97-107
88-99
Average (%)
95
100
95
1:4
Range (%)
95-109
89-97
97-104
Average (%)
103
92
101
1:8
Range (%)
93-103
98-110
99-108
Average (%)
97
104
104
1:16
Range (%)
95-102
85-98
93-105
Average (%)
99
93
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
98
EDTA plasma (n=5)
94-106
99
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
8.68
24.8
85.98
9.97
24.1
80.56
Standard deviation
0.51
1.19
4.32
0.53
1.1
3.87
C V (%)
5.93
4.81
5.02
5.3
4.58
4.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CTSB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTSB in samples. No significant cross-reactivity or interference between Human CTSB and analogues was observed.
