The Human CXCR3 (Chemokine CXC Receptor 3) CLIA Kit is a precision instrument designed for the accurate detection of CXCR3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, guaranteeing dependable and consistent results, making it perfect for a wide array of research applications.CXCR3 is a critical chemokine receptor involved in inflammatory responses and immune cell trafficking.
It plays a pivotal role in various diseases including autoimmune disorders, inflammatory diseases, and cancer, making it a valuable biomarker for understanding disease pathology and developing potential treatments.Invest in the Human CXCR3 CLIA Kit to enhance your research capabilities and uncover new insights into the mechanisms underlying various diseases. Get accurate and reliable results every time with this cutting-edge kit.
Product Name:
Human CXCR3 (Chemokine CXC Receptor 3) CLIA Kit
SKU:
HUES00515
Target:
Human CXCR3 (Chemokine CXC Receptor 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CXCR3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CXCR3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CXCR3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CXCR3. You can calculate the concentration of Human CXCR3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
96-113
93-108
Average (%)
91
104
101
1:4
Range (%)
101-115
93-107
102-115
Average (%)
109
99
109
1:8
Range (%)
96-113
95-109
92-108
Average (%)
103
102
100
1:16
Range (%)
103-120
85-98
97-114
Average (%)
110
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
100
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
100-114
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.69
194.55
842.02
110.24
180.0
856.08
Standard deviation
11.18
18.4
56.67
9.89
15.14
88.09
C V (%)
10.1
9.46
6.73
8.97
8.41
10.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CXCR3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CXCR3 in samples. No significant cross-reactivity or interference between Human CXCR3 and analogues was observed.
